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- Princess Peach as Penelope Pitstop
- Smithy as The Hooded Claw
- Wario and Waluigi as The Bully Bros.
- Mushroom King as Clyde
- Baby Mario as Snoozy
- Baby Luigi as Zippy
- Donkey Kong Jr. as Yak Yak
- Baby Yoshi as Dum-Dum
- Conker (From Conker's Bad Fur Day) as Softy
- Bowser Jr. as Pockets
- Kazooie (From Banjo-Kazooie) as Chug-a-Boom