• Mario as Calvin
  • Luigi as Charles
  • Wario as Ryan
  • Waluigi as Boo
  • Yoshi as Josh
  • Toad as Samson
  • Donkey Kong as Chief
  • Funky Kong as Sunny
  • Red Yoshi as Bully
  • Yellow Yoshi as Zachary
  • Oogtar as Principal Sands
  • Peach as Lauren
  • Birdo as Mrs. Murray
  • Daisy as Mrs. Crapple
  • Larry "Cheatsy" Koopa as Dr. Newman
  • Lemmy "Hip" Koopa as Paramedic #1
  • Iggy "Hop" Koopa as Paramedic #2
  • Wendy "Kootie Pie" O Koopa as Elderly Woman
  • Ludwig "Kooky" Von Koopa as Bus Driver
  • Candy Kong as Neighbor
  • Birdo as Receptionist
  • Toadette as Nurse
  • Chunky Kong as Calvin's Classmate

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