• Luigi as Miguel
  • Mario as Tulio
  • Princess Daisy as Chel
  • Funky Kong, Wario, Waluigi, Diddy Kong, Yoshi, Princess Rosalina, Princess Peach, Chunky Kong, Cranky Kong, Toadbert, Mallow, Bommer, Fleep, Dashshell, Piccolo and Oogtar as El Dorado Villagers
  • Color Toads as Big Mans
  • Donkey Kong as Altivo
  • Lemmy "Hip" Koopa, Iggy "Hop" Koopa and Larry "Cheasty" Koopa as Tzekel-Khan
  • Bowser Jr as Cortes
  • Smithy as Statue Jaguar
  • Tumble as Bibo the Armadillo
  • Baby Mario and Toad as Two Kids
  • Candy Kong and Birdo as Two Girls in Barcelona
  • Mr. L as Spanish Guard

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