Mario/Theodore Tugboat are parodies with Theodore Tugboat sounds and Mario, Sonic the Hedgehog and Thomas the Tank Engine and Friends clips.
- Mario as Theodore
- Yoshi as Hank
- Donkey Kong as George
- Luigi as Foduck
- Peach as Emily
- Toadsworth as The Dispatcher
- Waluigi as Oliver
- Lemmy "Hip" Koopa as Philip
- Iggy "Hop" Koopa as Fhillmore
- Larry "Cheasty" Koopa as Cocomagh
- Ludwig "Kooky" Von Koopa as Bobby
- Daisy as Rebecca
- Red Toad as Shelburne
- Toad as Digby
- Banjo as Bedford
- Chunky Kong as Inverness
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