• Mario as Thomas
  • Chunky Kong as Henry
  • Donkey Kong as Gordon
  • Oogtar as James
  • Yoshi as Percy
  • Toad as Toby
  • Bowser as Diesel 10
  • Larry Koopa as Splatter
  • Roy Koopa as Dodge
  • Rosalina as Lady
  • Edward as Mr. Counductor
  • Thomas as Mr. C Junior
  • Emily as Stacey Jones
  • Gordon as Billy Twofeathers
  • Toby as Burnett Stone
  • Rosie as Lily
  • Percy as Patch
  • Molly as Lily's Mom
  • Bertie as Mutt
  • Oliver as The Train Announcer

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