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- Mickey Mouse (From Mickey Mouse) as Thomas
- Amelia and Agabli Gabbles (from The Aristocats) as Annie and Clarabel
- Piglet (From Winnie the Pooh) as Percy
- Donald Duck (From Mickey Mouse) as James (No Talking Role)
- Goofy (From Mickey Mouse) as Bertie
- Various Passengers as Themselves
- An Animator as Thomas' Driver
- Walt Disney as Sir Topham Hatt
- Monkeys (From The Jungle Book) as Troublesome Trucks