- Mickey Mouse as Crash Bandicoot
- Minnie Mouse as Coco Bandicoot
- Casey Junior as Crunch Bandicoot
- Ludwig Von Drake as Aku Aku
- Captain Hook (from Peter Pan) as Dr. Neo Cortex
- Mr. Smee (from Peter Pan) as Dr N. Gin
- Horace Horsecollar as N Trophy
- Daisy Duck as Pasadena O Possum
- The Mad Doctor as Dingodile
- Chip and Dale as The Evil Twins
- Scrooge McDuck as Von Clutch
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