• Mickey Mouse as Alex
  • Goofy as Marty
  • Donald Duck as Melman
  • Daisy Duck as Gloria
  • Darkwing Duck (from Darkwing Duck) as Skipper
  • Launchpad McQuack (from Ducktales) as Kowalski
  • Honker Muddlefoot (from Darkwing Duck) as Rico
  • Gosalyn Mallard (from Darkwing Duck) as Private
  • Panchito Pistoles as King Julien
  • Jose Carioca as Maurice
  • Max as Mort
  • Chip and Dale as Mason and Phil
  • Pete as Makunga
  • Willie the Giant as Teetsi
  • Sora (from Kingdom Hearts) as Zuba
  • Kairi (from Kingdom Hearts) as Florrie
  • Minnie Mouse as Gia
  • Scrooge McDuck as Vitaly
  • Ludwing Von Drake as Stefano
  • Witch Hazel as Du Bois

Voice Cast:

  • Ben Stiller as Mickey
  • Chris Rock as Goofy
  • David Schwimmer as Donald
  • Jada Pinkett Smith as Daisy
  • Tom McGrath as Darkwing
  • Chris Miller as Launchpad
  • John DiMaggio as Honker
  • Christopher Knights as Gosalyn
  • Sacha Baron Cohen as Panchito
  • Cedric the Entertainer as Jose
  • Andy Ritcher as Max
  • Conrad Vernon as Chip (Dale is unvoiced)
  • Alec Baldwin as Pete
  • Fred Tatasciore as Willie the Giant
  • Bernie Mac as Sora
  • Sherri Shepherd as Kairi
  • Jessica Chastain as Minnie
  • Bryan Canstron as Scrooge McDuck
  • Martin Short as Ludwing Von Drake
  • Frances McDormand as Witch Hazel

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