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Mickey Mouse/Muppets is a parody of the Muppets by Jim Henson.
- Mickey Mouse as Kermit the Frog
- Minnie Mouse as Miss Piggy
- Goofy as Fozzie Bear
- Donald Duck as The Great Gonzo (Donald and Gonzo are both Mickey and Kermit's friends who sometimes get into trouble)
- Stitch (from Lilo and Stitch) as Animal
- Daisy Duck as Camilla
- Slinky Dog (from Toy Story) as Rowlf the Dog
- Jiminy Cricket (from Pinocchio) as Scooter
- Genie (from Aladdin) as Dr. Teeth
- Horace Horsecollar as Floyd Pepper
- Clarabelle Cow as Janice
- Launchpad McQuack as Zoot
- Yen Sid as Floyd Pepper
- The Troubadour as Bunsen
- Ludwig Von Drake as Beaker
- Chief O'Hara as The Chef
- Pete as Statler
- Julius as Waldorf