• Mickey Mouse as Peter Rabbit
  • Minnie Mouse as Jemima Puddle-Duck
  • Clarabelle Cow as Mrs. Tiggy-Winkle
  • Donald Duck as Benjamin Bunny
  • Daisy Duck as Flospy
  • Scrooge McDuck as Kep
  • Goofy as Mr. Jeremy Fisher
  • Pete as Mr. McGregor

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