- Mickey Mouse as Thomas
- Goofy as Edward
- Donald Duck as Henry
- Scrooge McDuck as Gordon
- Bart (from The Simpsons) as James
- Brian (from Family Guy) as Percy
- Horace Horsecollar as Toby
- Daisy Duck as Emily
- Minnie Mouse as Rosie
- Clarabel Cow as Molly
- Pluto as Charlie
- Pete as Spencer
- Sea Rogue (from Salty's Lighthouse) as Diesel
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