- Stuart as Sawyer
- Jorge as Hazel
- Scarlet as Lorraine
- Herb as Reed
- Kevin as Dr. Clay
- Tim as Dr. McCarthy
- Margo (from Despicable Me) as Julia
- Tom as Kyle
- Audrey (from Dr. Seuss' The Lorax) as Rebecca
- Gru as Troy
- Lucy Wilde (from Despicable Me 2) as Winter
- Mark as Hope
- T-Rex as Rufus
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