Cast[]
- Ghoulia Yelps as Twilight Sparkle
- Draculaura as Pinkie Pie
- Spectra Vondergeist as Rainbow Dash
- Clawdeen Wolf as Sunset Shimmer
- Frankie Stein as Fluttershy
- Lagoona Blue as Applejack
- Cleo De Nile as Rarity
- Red (from M&M's) as Spike
- Heath Burns as Flash Sentry
- Avea as Adagio Dazzle (both starts with "A")
- Bonita as Aria Blaze (both ends with "A")
- Sirena as Sonata Dusk (both starts with "S")
- Morgana McQuack (from Darkwing Duck) as Princess Celestia
- Isabella (from Scooby Doo and the Ghoul School) as Princess Luna
- Marceline (from Adventure Time) as Princess Cadence
- Count Spankulot (from Codename: Kids Next Door) as Shining Armor
- Darkwarrior Duck (from Darkwing Duck) and Mrs. Frizzle (from Magic School Bus) as Twilight's Parents
- One (from Seven Little Monsters) as Diamond Tiara
- Six (from Seven Little Monsters) as Silver Spoon
- Deuce Gorgon as Snips
- Gil as Snails
- Mistress Bloodgood as Miss Cheerlie
- Clawd Wolf as Big Macintosh
- Winnie (from Scooby Doo and the Ghoul School) as Apple Bloom
- Mona the Vampire as Sweetie Belle
- Sari Sumdac (from Transformers: Animated) as Scootaloo
- Nile (from Mummy Nanny) and Prowl (from Transformers: Animated) as Rarity's Parents
- Megavolt (from Darkwing Duck) as Discord
- Magica De Spell (from Ducktales) as Nightmare Moon
- Quackerjack (from Darkwing Duck) as King Sombra
- Negaduck (from Darkwing Duck) as Lord Tirek
- Crazy Old Cat Lady (from Codename: Kids Next Door) as Granny Smith
- Pistol Pete (from Goof Troop) as Babs Seed
- Mrs. Twombly (from Littlest Pet Shop) as Zecora