- John Smith as Mario
- Jane Smith as Princess Rosalina
- Eddie as Luigi
- Benjamin Danz as Toad
- Jasmine as Princess Peach
- Father as Yoshi
- Martin Coleman as Toadsworth
- Suzy Coleman as Princess Daisy
- Gwen as Birdo
- Julie as Toadette
- Jade as Toadiko
- Dr. Wexler as Wario
- Mr. Smith's Boss as Wendy O. Koopa (voice) (uncredited)
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