- Mr. Quincy Magoo as Mario
- Luanne LeSeur as Wendy O. Koopa
- Waldo Magoo as Luigi
- Bob Morgan as Toad
- FBI Agent Chuck Stupak as Yoshi
- CIA Agent Gustav Anders as Wario
- Stacey Sampanahodrita as Birdo
- Austin Cloquet as Bowser
- Ortega Peru as Bowser Jr.
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