- Wyatt Earp as Arthur
- Dr. John Henry "Doc" Holiday as Buster
- Clementine Carter as Francine
- Chihuahua as D.W.
- Newman Haynes Clanton as David
- Virgil Earp as George
- Morgan Earp as Brain
- Ike Clanton as Mr. Morest
- Billy Clanton as Pal
- Granville Thorndyke as Grandpa Dave
- Mayor as Mr. Haney
- Kate Nelson as Jane
- Mac the Barman as Mr. Ratburn
- John Simpson as Ed Crosswire
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