• Wyatt Earp as Mario
  • Dr. John Henry "Doc" Holiday as Luigi
  • Clementine Carter as Princess Peach
  • Chihuahua as Princess Daisy
  • Newman Haynes Clanton as Toad
  • Virgil Earp as Yoshi
  • Morgan Earp as Oogtar
  • Ike Clanton as Wario
  • Billy Clanton as Waluigi
  • Granville Thorndyke as Nabbit
  • Mayor as Toadsworth
  • Kate Nelson as Toadette
  • Mac the Barman as Donkey Kong
  • John Simpson as Diddy Kong

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