- Eliza Doolittle as Princess Peach
- Professor Henry Higgins as Mario
- Alfred P. Doolittle as Luigi
- Colonel Hugh Pickering as Toad
- Mrs. Higgins as Princess Daisy
- Freddy Eynsford-Hill as Yoshi
- Zoltan Karpathy as Donkey Kong
- Mrs. Pearce as Princess Rosalina
- Mrs. Eynsford-Hill as Pauline
- The Butler as Diddy Kong
- Cockney Bystander as Oogtar (uncredited)
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