• Eliza Doolittle as Princess Peach
  • Professor Henry Higgins as Mario
  • Alfred P. Doolittle as Luigi
  • Colonel Hugh Pickering as Toad
  • Mrs. Higgins as Princess Daisy
  • Freddy Eynsford-Hill as Yoshi
  • Zoltan Karpathy as Donkey Kong
  • Mrs. Pearce as Princess Rosalina
  • Mrs. Eynsford-Hill as Pauline
  • The Butler as Diddy Kong
  • Cockney Bystander as Oogtar (uncredited)

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