Cast[]
- Twilight Sparkle as Flik
- Princess Celestia as Princess Atta
- Apple Bloom as Dot
- Princess Luna as The Queen
- Spike as Aphie
- King Sombra as Hopper
- Starlight Glimmer as Molt
- Discord as Thumper
- Flim and Flam as Axel and Loco
- The Diamond Dogs as The Fly Brothers
- The Changelings as The Grasshoppers
- Nurse Redheart as Dr Flora
- Derpy as Mr Soil
- Cozy Glow as Thorny
- Queen Chrysalis as Cornelius
- Rarity as Rosie
- Scootaloo and Sweetie Belle as Tuck and Roll
- Rainbow Dash as Francis
- Pinkie Pie as Hemilich
- Big Macintosh as Dim
- Fluttershy as Slim
- Miss Cheerilee as Gypsy
- Applejack as Manny
- Dr. Whooves as P.T Flea