Cast
- Rainbow Dash as Numbuh 1/Female Numbuh 1
- Pinkie Pie as Numbuh 2/Female Numbuh 2
- Fluttershy as Numbuh 3/Male Numbuh 3
- Applejack as Numbuh 4/Female Numbuh 4
- Rarity as Numbuh 5/Male Numbuh 5
- Twilight Sparkle as Numbuh 362/Dragon Numbuh 362/Male Dragon Numbuh 362/Numbuh 6
- Starlight Glimmer as Jessica/Numbuh 7
- Spike as Tommy Gilligan
- Trixie as Lizzie
- Discord as Cree Lincoln
- Discord as Father
- The Diamond Dogs as the DCFDTL
- Ahuizotl as Heinrich von Marzipan
- Shining Armor as Numbuh 60
- Flash Sentry as Numbuh 274
- Princess Celestia as Numbuh 86
- Spitfire as Numbuh 10
- Sweetie Belle as Mushi Sanban
- Princess Luna as Numbuh 999
- King Sombra as Grandfather
- Hydra as Chad Dickson
- Daring Do as Vin Moosk
- Garble as Windsor
- Flim and Flam as Mr. Wink and Mr. Fibb
- Queen Chrysalis as Count Spankulot
- Big Mac as Mr. Fizz