Version 1[]
- Rainbow Dash as Batman/Bruce Wayne
- Pinkie Pie as the Joker
- Scootaloo as Robin/Dick Grayson
- Twilight Sparkle as Batgirl/Barbara Gordon
- Randolph as Alfred Pennyworth
- Cheese Sandwich as Harley Quinn
- Applejack as Scarecrow
- Mayor Mare as Mayow McCaskill
- Bill Neigh as The Riddler
- Lord Tirek as Bane
- Pilot Pony #1 as Pilot Bill
- Filthy Rich as Two-Face
- Rarity as Catwoman
- The Storm King as Clayface
- Fluttershy as Poison Ivy
- Pilot Pony #2 as Captain Dale