- Jenny Johnson as Princess Peach
- Matthew "Matt" Saunders as Mario
- Hannah Lewis as Birdo
- Professor Bedlam as Bowser
- Vaughn Haige as Luigi
- Carla Dunkirk as Princess Daisy
- Leo as Yoshi
- Lenny as Donkey Kong
- Steve Velard as Wario
- Young Barry Lambert as Baby Mario
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