- Jenny Johnson as Miss Piggy
- Matthew "Matt" Saunders as Kermit the Frog
- Hannah Lewis as Annie Sue Pig
- Professor Bedlam as Uncle Deadly
- Vaughn Haige as Gonzo the Great
- Carla Dunkirk as Camilla the Chicken
- Leo as Rizzo the Rat
- Lenny as Sam the Eagle
- Steve Velard as Dr. Bunsen Honeydew
- Young Barry Lambert as Beaker
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