- Huxley Pig as Professor Ratigan
- Sooty as Fidget
- Pingu as Bartholomew
- Meg Griffin as Felisha
- Fireman Sam as Basil
- Oakie Doke as Dr. Dawson
- Wallace as Hiram Flaversham
- Noddy as Olivia Flaversham
- Numbuh 5 as Queen Mousetoria
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