- Nancy Drew as Princess Daisy
- Corky Veinshtein as Mario
- Ned Nickerson as Luigi
- Jane Brighton as Princess Peach
- Carson Drew as Toad
- John Leshing as Yoshi
- Dehlia Draycott as Princess Rosalina
- Dashiel Zachary Biedermeyer as Wario
- Inga Veinshtein as Toadette
- Trish as Birdo
- Barbara Barbara as Wendy O. Koopa
- Sgt. Billings as Donkey Kong
- and more
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