- Rose Loomis as Annie Sue Pig
- George Loomis as Kermit the Frog
- Polly Cutler as Gonzo the Great
- Ray Cutler (billed as Casey Adams) as Rizzo the Rat
- Inspector Starkey as Statler
- Patrick as Waldorf
- Mr. Kettering as Beaker
- Mrs. Kettering as Miss Piggy
- Mr. Qua as Dr. Bunsen Honeydew
- Boatman as The Newsman
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