- Yoshi (from Mario) as Scooby Doo
- Luigi (from Mario) as Shaggy Rogers
- Mario (from Mario) as Fred Jones
- Peach (from Mario) as Daphne Blake
- Daisy (from Mario) as Velma Dinkley
- Kirby (from Kirby) as Scrappy Doo
- Bowser (from Mario) as Villain
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