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- Noddy as Basil of Baker Street
- Young Simba (from The Lion King) as Dr. Dawson
- Marie (from The Aristocats) as Olivia Flaversham
- Thomas O'Malley (from The Aristocats) as Hiram Flaversham
- Shere Khan (from The Jungle Book) as Professor Ratigan
- Kaa (from The Jungle Book) as Fidget the Bat
- Pumbaa (from The Lion King) as Toby the Dog
- The Trucks (from Thomas & Friends) as Ratigan's Henchmen
- Sly as Bill the Lizard
- Martha Monkey as Felicia the Cat
- Master Tubby Bear as Bartholomew
- Tessie Bear as The Queen Mouse