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- Mr. Wobbly Man as Professor Ratigan
- Sammy Sailor as Fidget
- Bert Monkey as Bartholomew
- Martha Monkey as Felisha
- Noddy as Basil
- Mr. Milko as Dr. Dawson
- Big Ears as Hiram Flaversham
- Tessie Bear as Olivia Flaversham
- Dinah Doll as Queen Mousetoria