- Gabey as Mario
- Chip as Yoshi
- Ozzie as Luigi
- Claire Huddesen as Princess Peach
- Brunhilde Esterhazy as Birdo
- Ivy Smith as Princess Daisy
- Madame Dilyovska as Pauline
- Lucy Schmeeler as Princess Rosalina
- Professor as Diddy Kong
- Francois as Wario
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