- Thomas as Kermit the Frog
- Oliver as Dr. Bunsen Honeydew
- Sir Topham Hatt as Jim Henson
- Annie and Clarabel as the Snowths
- Troublesome Trucks as Chickens and Penguins
- Spiteful Brakevan as Timmy Monster
- Old Slow Coach as Mildred Huxtetter
- Terence as Robin the Frog
- Caroline as Annie Sue Pig
- Horrid Lorries as Mutations
- Thumper as Pepe the King Prawn
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