• Connie Abbott as Minnie Mouse
  • Bill Abbott as Mickey Mouse
  • Gene Morrison as Professor Ludwig Von Drake
  • Jaynie as Daisy Duck
  • Natalie as Clarabelle Cow
  • Sinjin as Donald Duck
  • Elsie as Ortensia
  • Gene Morrison Orchestra as Goofy
  • The Modernaries as Clara Cluck, Huey Duck, Louie Duck, Dewey Duck, and Pluto
  • The Nicholas Brothers as Horace Horsecollar and Scrooge McDuck
  • Bobby Hackett as Oswald the Lucky Rabbit

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