- Connie Abbott as Minnie Mouse
- Bill Abbott as Mickey Mouse
- Gene Morrison as Professor Ludwig Von Drake
- Jaynie as Daisy Duck
- Natalie as Clarabelle Cow
- Sinjin as Donald Duck
- Elsie as Ortensia
- Gene Morrison Orchestra as Goofy
- The Modernaries as Clara Cluck, Huey Duck, Louie Duck, Dewey Duck, and Pluto
- The Nicholas Brothers as Horace Horsecollar and Scrooge McDuck
- Bobby Hackett as Oswald the Lucky Rabbit
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