- Oswald as Arthur
- Laverne as D.W.
- Steve Tree as David Read
- Madame Butterfly as Jane Read
- Catrina as Baby Kate
- Weenie as Pal
- Henry as Buster
- Daisy as Francine
- Bingette Bunny as Muffy
- Johnny Snowman as The Brain
- Pongo as Binky
- Buster the Turtle as George
- Flippy as Sue Ellen
- Eggbert & Leo as The Tibble Twins
- Andy Pumpkin as Oliver Frensky
- Bingo Bunny as Ed Crosswire
- Sammy Starfish as Mr. Ratburn
- Woodrow as Spanky
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