- Moses "Moze" Pray as Luigi
- Addie Loggins as Mario
- Trixie Delight as Princess Daisy
- Deputy Hardin/Jess Hardin as Donkey Kong
- Floyd as Toad
- Imogene as Yoshi
- The Minister as Diddy Kong
- Mr. Robertson as Wario
- The Widow Bates (Marie) as Pauline
- Leroy as Oogtar
- 2nd Deputy as Bowser
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