- Spongebob (from Spongebob) as Dr. Clay
- Sandy (from Spongebob) as Lorraine
- Stanley as Sawyer
- Mindy (from Spongebob) as Hazel
- Dr. Benedict (from Recess) as Reed
- Patrick (from Spongebob) as Dr. McCarthy
- Mr. Krabs (from Spongebob) as Caleb
- Mrs. Puff (from Spongebob) as Julia
- Stella (from Over the Hedge) as Mandy
- Pat as Kyle
- TJ (from Recess) as Troy
- Spinelli (from Recess) as Winter
- Eleanor (from Alvin and the Chipmunks) as Hope (dolphin)
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