- Jack Ryan as Mario
- Dr. Caroline Catherine Ryan as Birdo
- Kevin O'Donnell as Yoshi
- Sean Miller as Bowser
- Sally Ryan as Princess Peach
- Lord William Holmes as Wario
- Rose as Princess Daisy
- Lieutenant Commander Robby Jackson as Toadsworth
- Marty Cantor as Donkey Kong
- Vice Admiral Jim Greer as Geno
- Paddy O'Neil as Toad
- Dennis Cooley as Luigi
- Sgt. Owens as Mighty Plumber
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