- First Lieutenant Rafe McCawley as Bugs Bunny
- Daniel "Danny" Walker as Road Runner
- Nurse Evelyn Johnson as Lola Bunny
- Pretty Officer Second Class Dorie Miller as Pepe Le Pew
- Earl as Sylvester
- President Franklin D. Roosevelt as Elmer Fudd
- Admiral Husband E. Kimmel as Tax
- Kaigun Taisho Isoroku Yamamoto as Speedy Gonzales
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