- First Lieutenant Rafe McCawley as Mickey Mouse
- Daniel "Danny" Walker as Horace Horsecollar
- Nurse Evelyn Johnson as Minnie Mouse
- Pretty Officer Second Class Dorie Miller as Professor Ludwig Von Drake
- Earl as Pete
- President Franklin D. Roosevelt as Mortimer Mouse
- Admiral Husband E. Kimmel as Goofy
- Kaigun Taisho Isoroku Yamamoto as Pluto
- Major Jimmy Doolittle as Chip
- First Lieutenant Billy Thompson as Dale
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