• First Lieutenant Rafe McCawley as Mickey Mouse
  • Daniel "Danny" Walker as Horace Horsecollar
  • Nurse Evelyn Johnson as Minnie Mouse
  • Pretty Officer Second Class Dorie Miller as Professor Ludwig Von Drake
  • Earl as Pete
  • President Franklin D. Roosevelt as Mortimer Mouse
  • Admiral Husband E. Kimmel as Goofy
  • Kaigun Taisho Isoroku Yamamoto as Pluto
  • Major Jimmy Doolittle as Chip
  • First Lieutenant Billy Thompson as Dale

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