- First Lieutenant Rafe McCawley as Kermit the Frog
- Daniel "Danny" Walker as Fozzie Bear
- Nurse Evelyn Johnson as Miss Piggy
- Pretty Officer Second Class Dorie Miller as Jim Henson
- Earl as Animal
- President Franklin D. Roosevelt as Uncle Deadly
- Admiral Husband E. Kimmel as Sam the Eagle
- Kaigun Taisho Isoroku Yamamoto as Robin the Frog
- Major Jimmy Doolittle as Crazy Harry
- First Lieutenant Billy Thompson as Scooter
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