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- Phineas as Peter Rabbit
- Ferb as Benjamin Bunny
- Isabella as Jemima Puddle Duck
- Baljeet as Tom Kitten
- Candace as Mrs. Tiggy Winkle
- Jeremy as Mr. Jeremy Fisher
- Stacy as Flopsy
- Vanessa as Mrs. Tittlemouse
- Major Monogram as Mr. Bouncer
- Perry The Platypus as Kep
- Buford as Samuel Whiskers
- Dr. Heinz Doofenshmirtz as Mr. McGregor
- Suzy as Mrs. McGregor