• Beca Mitchell as Pauline
  • Jesse Swanson as Mario
  • Aubrey Posen as Princesss Peach
  • Chloe Beale as Princess Daisy
  • Fat Amy/Patricia as Princess Rosalina
  • Stacie Conrad as Birdo
  • Lilly Okanakamura as Toadette
  • Benji Applebaum as Luigi
  • Bumper Allen as Yoshi
  • Donald as Toad
  • Luke as Wario
  • Kimmy-Jin as Wendy O. Koopa
  • Tommy as Toadsworth
  • John Smith as Donkey Kong
  • Dr. Mitchell as Diddy Kong

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