- Beca Mitchell as Miss Piggy
- Jesse Swanson as Kermit the Frog
- Aubrey Posen as Janice
- Chloe Beale as Annie Sue Pig
- Fat Amy/Patricia as Miss Mousey
- Stacie Conrad as Wanda
- Benji Applebaum as Gonzo the Great
- Bumper Allen as Jim Henson
- Donald as Rizzo the Rat
- Luke as Scooter
- Dr. Mitchell as Beaker
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