• William Frederick Cody as Mario
  • Evelyn Hastings as Princess Peach
  • Wild Bill Hickock as Luigi
  • Denny Russell as Birdo
  • Rance Hastings as Yoshi
  • Jim Bridger as Diddy Kong
  • Red Barrett as Toad
  • Joe Cooper as Donkey Kong
  • Pemberton as Wario
  • Sergeant Russell as Toadsworth
  • Chief Yellow Hand as Mighty Plumber
  • Harvey as Mallow

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