- William Frederick Cody as Mario
- Evelyn Hastings as Princess Peach
- Wild Bill Hickock as Luigi
- Denny Russell as Birdo
- Rance Hastings as Yoshi
- Jim Bridger as Diddy Kong
- Red Barrett as Toad
- Joe Cooper as Donkey Kong
- Pemberton as Wario
- Sergeant Russell as Toadsworth
- Chief Yellow Hand as Mighty Plumber
- Harvey as Mallow
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