- Marshal Howard Nightingale as Mickey Mouse
- Jack Strawhorn as Professor Ludwig Von Drake
- Wesley as Dewey Duck
- Hellman as Donald Duck
- Krag as Huey Duck
- Pensteman as Louie Duck
- Peppe as Jiminy Cricket
- Mrs. Cooper as Daisy Duck
- Mr. Cooper as Horace Horsecollar
- Wiley as Pete
- Laurie as Minnie Mouse
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