- Rebecca Winstead as Princess Daisy
- Tony Kent as Yoshi
- Harry Kipper as Mario
- Miranda as Princess Rosalina
- Gwen as Toadette
- Aloysius as Toadbert
- Homer Busby as Donkey Kong
- Orville Smithers as Luigi
- Lola Lee as Birdo
- Purvis as Wario
- An Organist at a Radio Station as Diddy Kong
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