- Rocko as Mario (both are the main protagonists)
- Heffer as Yoshi (both are the best friends)
- Filburt as Luigi (both are strange and timid)
- Shiela as Peach
- Ed Bighead as Bowser (both are angry and mean)
- Dr. Bendova as Dr. Mario
- Claudette as Rosalina
- Ralph Bighead as Bowser Jr.