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- Olie as Bugs Bunny
- Zowie as Lola Bunny
- Spot as Road Runner
- Baby Billy as Tweety
- Olie's Dad as Sylvester
- Billy as Daffy Duck
- Olie's Mom as Granny
- Pappy as Pepe Le Paw
- Screwy as Wile E. Coyote
- Pollie Pi as Tina Duck
- Uncle Gizmo as Foghorn Leghorn
- Gene Littlegreen as Elmer Fudd
- Junior Littlegreen as Porky Pig