- Tommy Pickles as Scooby-Doo
- Chuckie Finster as Shaggy
- Drew Pickles as Fred
- Didi Pickles as Daphne
- Kimi as Lena Dupree
- Dil Pickles as Scrappy Doo
- Lil DeVille as Scooby Dum
- Phil DeVille as Buttercup (Powerpuff Girls)
- Reptar as Villian
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