• Mario as Woody
  • Yoshi as Buzz Lightyear
  • Peach as Jessie
  • Shrek as The Prospector
  • Black Yoshi as Mr. Potato Head
  • Molly (from TTTE) as Mrs. Potato Head
  • Bowser Junior as Rex
  • Donkey Kong as Hamm
  • Luigi as Slinky Dog
  • Minnie Mouse as Bo Peep
  • Woody as Bullseye
  • Potato as Wheezy
  • Wario as Sarge
  • The Fat Controller (from TTTE) as Andy Davis
  • Baby Chico (from Handy Manny) as Molly Davis
  • Elmo Monster as Al McWhiggin
  • Know-It-All Boy (from The Polar Express) as Buster
  • Wario's Toys as Sarge's Soldiers
  • Bowser as Evil Emperor Zurg
  • Bowser's Guards as Zurgbots
  • The tourguide as Pilot
  • Yellow Yoshi as Flik (from A Bug's Life)
  • Chef Pee Pee as Heimlich (from A Bug's Life)

​Voice Cast

  • Tom Hanks as Mario
  • Tim Allen as Yoshi
  • Joan Cusack as Peach
  • Kelsey Grammer as Shrek
  • Don Rickles as Black Yoshi
  • Estelle Harris as Molly (from TTTE)
  • Wallace Shawn as Barney
  • John Ratzenberger as Donkey Kong
  • Jim Varney as Luigi
  • Annie Potts as Minnie Mouse
  • Frank Welker as Woody/Know-It-All Boy (from The Polar Express)
  • Joe Ranft as Potato/Chef Pee Pee
  • R. Lee Ermey as Wario
  • John Morris as The Fat Controller (from TTTE)
  • Wayne Knight as Elmo Monster
  • Andrew Stanton as Bowser
  • Jim Hanks as The tourguide
  • Dave Foley as Yellow Yoshi

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