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- Samurai Jack (from Samurai Jack) as Mario
- Ben Tennyson (from Ben 10: Ultimate Alien) as Luigi
- Flapjack (from The Marvelous Misadventures of Flapjack) as Toad
- Snow White (from Snow White And The Seven Dwarfs) as Princess Toadstool
- Koala Kong (from Crash Bandicoot) as King Koopa